Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Primer Sequences Used for Detection Extended-Spectrum Beta-Lactamase and Carbapenemase Genes

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques: Sequencing, Amplification

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Magnitude and Distribution of Carbapenemase Producing Enterobacteriaceae Isolates from Patients with BSI at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Comparison of Antimicrobial Resistance Patterns Between ESBLand Carbapenemase Producing and Non-Producing Enterobacteriaceae Isolates at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques: Comparison

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Gel image of the ESBL and carbapenemase genes from Enterobacteriaceae isolates at TASH 2019. ( A ) bla CTX-M (754bp), bla SHV (403bp) and bla TEM (293bp).

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Distribution of ESBL and Carbapenemase Genes Among Enterobacteriaceae Isolates from Patients with BSI at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Scientific Reports

Article Title: A host-directed macrocyclic peptide therapeutic for MDR gram negative bacterial infections

doi: 10.1038/s41598-021-02619-y

Figure Lengend Snippet: MICs of MTD12813, RTD-1 and antibacterial drugs (μg/ml).

Article Snippet: In addition, 1.25 μg/ml MTD12813 was incubated with log-phase Kp -1705 or E. coli ATCC-BAA-2340 suspended in 50 mM HEPES at 37 °C for 24 h. Intact MTD12813 was quantified by LC–MS/MS which showed 72.4 ± 0.93% and 81.3 ± 2.1% of MTD12813 was found intact post Kp -1705 and E. coli incubations respectively.

Techniques:

Journal: Scientific Reports

Article Title: A host-directed macrocyclic peptide therapeutic for MDR gram negative bacterial infections

doi: 10.1038/s41598-021-02619-y

Figure Lengend Snippet: MTD12813 promotes survival in CRE bacteremic sepsis. ( a - c ) Mice were challenged i.p. with Kp -1705 (3 - 5 x 10 8 CFU) at t = 0. ( a ) One h post infection (p.i.) mice were treated i.p. with MTD12813 at 0.5, 1.25, 5 mg/kg, or saline vehicle and monitored for up to 28 d. ( b ) Mice were treated with a single i.p. injection of 1.25 mg/kg MTD12813 before (-1 or -3 h) or after (+1 or +3 h) infection; controls received saline. P values compared to saline control. ( c ) Mice were treated 1 h p.i. with a single i.p. dose of 1.25 mg/kg MTD12813, meropenem, imipenem, colistin, or polymyxin B or 5 mg/kg RTD-1. ( d ) Mice were challenged i.p. with CRE E. coli BAA-2340 (3 - 6 x 10 7 CFU). One h after infection, mice received a single i.p. injection of 5 or 1.25 mg/kg MTD12813 or saline. P values determined by Fisher’s exact test.

Article Snippet: In addition, 1.25 μg/ml MTD12813 was incubated with log-phase Kp -1705 or E. coli ATCC-BAA-2340 suspended in 50 mM HEPES at 37 °C for 24 h. Intact MTD12813 was quantified by LC–MS/MS which showed 72.4 ± 0.93% and 81.3 ± 2.1% of MTD12813 was found intact post Kp -1705 and E. coli incubations respectively.

Techniques: Infection, Saline, Injection, Control